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Quantitative fluorescence microscopy techniques to study three-dimensional organisation of T-cell signalling molecules.
Chum, Tomáš ; Cebecauer, Marek (advisor) ; Lánský, Zdeněk (referee) ; Brameshuber, Mario (referee)
10 SUMMARY Proteins represent one of the basic building blocks of all organisms. To understand their function at the molecular level is one the critical goals of current biological, biochemical and biophysical research. It is important to characterise all aspects that affect the localisation of proteins into different compartments with specific functions, the dynamic structure of proteins and their role in multiprotein assemblies, because altering these properties can lead to various diseases. Most of the proteomic studies are nowadays performed using biochemical approaches that allow us to study multicellular organism or tissue at once. The disadvantage of these methods is complex preparation of sample and the need for a large number of cells, which leads to the loss of information at the molecular level and in individual cells. On the contrary, microscopy can provide rather detailed information about proteins of interest and at the level of a single cell. A variety of fluorescence microscopy methods in combination with recombinant DNA techniques were applied to elucidate subcellular localisation of transmembrane adaptor proteins (TRAPs) in human lymphocytes and their nanoscopic organisation at the plasma membrane. Linker of activation of T lymphocytes (LAT), phosphoprotein associated with...
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Regulation of LAT trafficking to the plasma membrane
Rakhimbekova, Anastasia ; Cebecauer, Marek (advisor) ; Černý, Jan (referee)
Linker for activation of T cells is a palmitoylated transmembrane adaptor protein, which is expressed in most of immune cells, but not in immature and mature B cells. It plays an important role in T-cell activation and maturation. LAT is synthesized in the endoplasmic reticulum. Its sorting to the plasma membrane is controlled with various determinants, such as properties of the transmembrane domain and structural motifs in the intracellular part of the protein. Some of those determinants are important for posttranslational modifications, export from the Golgi apparatus and, probably, membrane microdomain targeting, while others interact with COPII machinery and mediate protein export from the endoplasmic reticulum or targeting to endosomes. Keywords: LAT, functional motifs, protein sorting, plasma membrane, Golgi apparatus
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Lesson Activity Toolkit in the Smart Notebook software for interactive board
HAJDUCH, Petr
Lesson Activity toolkit is a collection of mini applications (applets) in the Smart Notebook software. It serves for creating educational materials with the use of the interactive board. It is a flash application which can be loaded with an extra content. This Bachelor's thesis focuses on these applications - it will elaborate on how these activities are used in contemporary education, how to work with them and which modules are not present in the gallery and could expand it efficiently. It also examines the possibility of creating and translating these modules and their implementations into the SmartNotebook program interface.
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